Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology
Article Title: CRISPR/Cas9 Editing of Glia Maturation Factor Regulates Mitochondrial Dynamics by Attenuation of the NRF2/HO-1 Dependent Ferritin Activation in Glial Cells
doi: 10.1007/s11481-019-09833-6
Figure Lengend Snippet: Effect of GMF on MPP+ induced Ca2+ influx in BV2 microglial cells. The BV2 and BV2-G cells were seeded (3×106) in a 96-well plate. Then the cells were pre-stained using Fura-2 QBT Calcium Assay kit according to the kit manufacturer’s instruction. The cells were treated MPP+ (0.1 mM) and plates were transferred to a FLEX Station3 multi-mode benchtop scanning fluorometer chamber (Molecular Devices). Representative results show the effect of MPP+ induced Ca2+ mobilization (flux) which is significantly reduced in BV2-G cells as compared with BV2 microglial cells. Values are given as mean ± SEM of three experiments in each group and represented as arbitrary unit.
Article Snippet: The cells were treated MPP + (0.1 mM) and plates were transferred to a FLEX Station3 multi-mode benchtop scanning fluorometer chamber (Molecular Devices).
Techniques: Staining, Calcium Assay